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β catenin responsive firefly luciferase reporter plasmid top flash  (Addgene inc)


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    Addgene inc β catenin responsive firefly luciferase reporter plasmid top flash
    β Catenin Responsive Firefly Luciferase Reporter Plasmid Top Flash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2+catenin+reporter/TOP-GFP+(Plasmid+%2335489)/pmc12639897-241-11-20
    Average 93 stars, based on 22 article reviews
    β catenin responsive firefly luciferase reporter plasmid top flash - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Wnt5a is a crucial regulator of neurogenesis during cerebellum development
    Article Snippet: Dual Luciferase assay was done according to manufacturer’s protocol (Promega, Cat. no. E1910) using Luminometer (TD20/20, Promega). .. Briefly, primary cerebellar cells were electroporated with TOPFlash (Plasmid #12457, Addgene), β-catenin reporter with 8X TCF/LEF binding sites and FOPFlash (Plasmid #12456, Addgene), β-catenin reporter with 8X mutated TCF/LEF binding sites vector along with Renilla luciferase and were seeded in a 24 well plate . ..

    Article Title: Genetic Regulation of Retinal Development
    Article Snippet: The cells were then cotransfected 48 hours after plating with several plasmids using TurboFect (Thermo Fisher Scientific, #R0531). .. Each well received a combination of four types of plasmids: 1) a DIXDC1 expression plasmid (control, full length, truncated, or missense), 2) a 31 DVL2 expression plasmid (control or protein expressing), 3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash, Addgene, #12456), and 4) the transfection control Renilla luciferase-expression plasmid (pRL-null; Promega, #E2271). ..

    Article Title: PD-L1 interacts with Frizzled 6 to activate β-catenin and form a positive feedback loop to promote cancer stem cell expansion.
    Article Snippet: Cancer stem cells (CSCs) drive tumor initiation, progression, metastasis, and drug resistance.. We report here that programmed cell death ligand 1 (PD-L1) is constitutively expressed in cancer cells to maintain and expand CSC through a novel mechanism in addition to promoting cancer cell immune evasion.. We discovered that PD-L1 interacts with receptor Frizzled 6 to activate β-catenin signaling and increase β-catenin-targeted gene expression, such as a putative stem cell marker leucine-rich-repeatcontaining G-protein-coupled receptor 5.

    Article Title: Quantitative trait loci on chromosomes 9 and 19 modulate AII amacrine cell number in the mouse retina
    Article Snippet: Cells were grown in DMEM:F12 medium (#11320033; Thermo Fisher Scientific) with 20% Newborn Calf Serum (#160110167; Thermo Fisher Scientific) and penicillin/streptomycin (100 U/mL and 100 ug/mL, respectively; #15140122; Thermo Fisher Scientific) for 2 days, after which cells were transfected with a combination of plasmids using TurboFect (#R0531; Thermo Fisher Scientific). .. Each well received a combination of four types of plasmids: (1) a DIXDC1 expression plasmid (control, full-length, truncated, or missense), (2) a DVL2 expression plasmid (control or protein expressing), (3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash; RRID:Addgene_12456 ), and (4) the transfection control Renilla luciferase-expression plasmid (pRL-null; #E2271; Promega). ..

    Binding Assay:

    Article Title: Wnt5a is a crucial regulator of neurogenesis during cerebellum development
    Article Snippet: Dual Luciferase assay was done according to manufacturer’s protocol (Promega, Cat. no. E1910) using Luminometer (TD20/20, Promega). .. Briefly, primary cerebellar cells were electroporated with TOPFlash (Plasmid #12457, Addgene), β-catenin reporter with 8X TCF/LEF binding sites and FOPFlash (Plasmid #12456, Addgene), β-catenin reporter with 8X mutated TCF/LEF binding sites vector along with Renilla luciferase and were seeded in a 24 well plate . ..

    Luciferase:

    Article Title: Wnt5a is a crucial regulator of neurogenesis during cerebellum development
    Article Snippet: Dual Luciferase assay was done according to manufacturer’s protocol (Promega, Cat. no. E1910) using Luminometer (TD20/20, Promega). .. Briefly, primary cerebellar cells were electroporated with TOPFlash (Plasmid #12457, Addgene), β-catenin reporter with 8X TCF/LEF binding sites and FOPFlash (Plasmid #12456, Addgene), β-catenin reporter with 8X mutated TCF/LEF binding sites vector along with Renilla luciferase and were seeded in a 24 well plate . ..

    Article Title: Genetic Regulation of Retinal Development
    Article Snippet: The cells were then cotransfected 48 hours after plating with several plasmids using TurboFect (Thermo Fisher Scientific, #R0531). .. Each well received a combination of four types of plasmids: 1) a DIXDC1 expression plasmid (control, full length, truncated, or missense), 2) a 31 DVL2 expression plasmid (control or protein expressing), 3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash, Addgene, #12456), and 4) the transfection control Renilla luciferase-expression plasmid (pRL-null; Promega, #E2271). ..

    Article Title: Quantitative trait loci on chromosomes 9 and 19 modulate AII amacrine cell number in the mouse retina
    Article Snippet: Cells were grown in DMEM:F12 medium (#11320033; Thermo Fisher Scientific) with 20% Newborn Calf Serum (#160110167; Thermo Fisher Scientific) and penicillin/streptomycin (100 U/mL and 100 ug/mL, respectively; #15140122; Thermo Fisher Scientific) for 2 days, after which cells were transfected with a combination of plasmids using TurboFect (#R0531; Thermo Fisher Scientific). .. Each well received a combination of four types of plasmids: (1) a DIXDC1 expression plasmid (control, full-length, truncated, or missense), (2) a DVL2 expression plasmid (control or protein expressing), (3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash; RRID:Addgene_12456 ), and (4) the transfection control Renilla luciferase-expression plasmid (pRL-null; #E2271; Promega). ..

    Expressing:

    Article Title: Genetic Regulation of Retinal Development
    Article Snippet: The cells were then cotransfected 48 hours after plating with several plasmids using TurboFect (Thermo Fisher Scientific, #R0531). .. Each well received a combination of four types of plasmids: 1) a DIXDC1 expression plasmid (control, full length, truncated, or missense), 2) a 31 DVL2 expression plasmid (control or protein expressing), 3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash, Addgene, #12456), and 4) the transfection control Renilla luciferase-expression plasmid (pRL-null; Promega, #E2271). ..

    Article Title: Quantitative trait loci on chromosomes 9 and 19 modulate AII amacrine cell number in the mouse retina
    Article Snippet: Cells were grown in DMEM:F12 medium (#11320033; Thermo Fisher Scientific) with 20% Newborn Calf Serum (#160110167; Thermo Fisher Scientific) and penicillin/streptomycin (100 U/mL and 100 ug/mL, respectively; #15140122; Thermo Fisher Scientific) for 2 days, after which cells were transfected with a combination of plasmids using TurboFect (#R0531; Thermo Fisher Scientific). .. Each well received a combination of four types of plasmids: (1) a DIXDC1 expression plasmid (control, full-length, truncated, or missense), (2) a DVL2 expression plasmid (control or protein expressing), (3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash; RRID:Addgene_12456 ), and (4) the transfection control Renilla luciferase-expression plasmid (pRL-null; #E2271; Promega). ..

    Control:

    Article Title: Genetic Regulation of Retinal Development
    Article Snippet: The cells were then cotransfected 48 hours after plating with several plasmids using TurboFect (Thermo Fisher Scientific, #R0531). .. Each well received a combination of four types of plasmids: 1) a DIXDC1 expression plasmid (control, full length, truncated, or missense), 2) a 31 DVL2 expression plasmid (control or protein expressing), 3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash, Addgene, #12456), and 4) the transfection control Renilla luciferase-expression plasmid (pRL-null; Promega, #E2271). ..

    Article Title: Quantitative trait loci on chromosomes 9 and 19 modulate AII amacrine cell number in the mouse retina
    Article Snippet: Cells were grown in DMEM:F12 medium (#11320033; Thermo Fisher Scientific) with 20% Newborn Calf Serum (#160110167; Thermo Fisher Scientific) and penicillin/streptomycin (100 U/mL and 100 ug/mL, respectively; #15140122; Thermo Fisher Scientific) for 2 days, after which cells were transfected with a combination of plasmids using TurboFect (#R0531; Thermo Fisher Scientific). .. Each well received a combination of four types of plasmids: (1) a DIXDC1 expression plasmid (control, full-length, truncated, or missense), (2) a DVL2 expression plasmid (control or protein expressing), (3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash; RRID:Addgene_12456 ), and (4) the transfection control Renilla luciferase-expression plasmid (pRL-null; #E2271; Promega). ..

    Transfection:

    Article Title: Genetic Regulation of Retinal Development
    Article Snippet: The cells were then cotransfected 48 hours after plating with several plasmids using TurboFect (Thermo Fisher Scientific, #R0531). .. Each well received a combination of four types of plasmids: 1) a DIXDC1 expression plasmid (control, full length, truncated, or missense), 2) a 31 DVL2 expression plasmid (control or protein expressing), 3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash, Addgene, #12456), and 4) the transfection control Renilla luciferase-expression plasmid (pRL-null; Promega, #E2271). ..

    Article Title: Quantitative trait loci on chromosomes 9 and 19 modulate AII amacrine cell number in the mouse retina
    Article Snippet: Cells were grown in DMEM:F12 medium (#11320033; Thermo Fisher Scientific) with 20% Newborn Calf Serum (#160110167; Thermo Fisher Scientific) and penicillin/streptomycin (100 U/mL and 100 ug/mL, respectively; #15140122; Thermo Fisher Scientific) for 2 days, after which cells were transfected with a combination of plasmids using TurboFect (#R0531; Thermo Fisher Scientific). .. Each well received a combination of four types of plasmids: (1) a DIXDC1 expression plasmid (control, full-length, truncated, or missense), (2) a DVL2 expression plasmid (control or protein expressing), (3) the β-catenin reporter firefly luciferase-expressing plasmid (Super 8x TOPFlash; RRID:Addgene_12456 ), and (4) the transfection control Renilla luciferase-expression plasmid (pRL-null; #E2271; Promega). ..



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    Image Search Results


    HIF‐3α modulates the canonical Wnt/β pathway in a cell context‐dependent manner. Stable scrambled‐sh control and HIF‐3α‐sh RKO (A) or SW480 cells (B) were transiently transfected with the pTOPFlash or pFOPFlash (control) reporter plasmids. Twenty‐four hours posttransfection, SW480 cells were washed and lysed, and the luciferase activity was assayed (B). For RKO cells, which display normal canonical Wnt that needs to be activated by Wnt3a ligand (A), cells were serum starved (2%) for 10 h, and then Wnt3a (100 ng/mL) or vehicle was added to the medium to stimulate cells for 12 h period; the cells were then washed and lysed, and the luciferase activity was assayed. The activity in both cell types was normalized with respect to the activity of Renilla luciferase or with respect to the protein content in each sample. The data represent the means ± SEM from at least five independent assays. *** p < .001.

    Journal: Iubmb Life

    Article Title: Hypoxia inducible factor 3‐alpha promotes a malignant phenotype in colorectal cancer cells

    doi: 10.1002/iub.70007

    Figure Lengend Snippet: HIF‐3α modulates the canonical Wnt/β pathway in a cell context‐dependent manner. Stable scrambled‐sh control and HIF‐3α‐sh RKO (A) or SW480 cells (B) were transiently transfected with the pTOPFlash or pFOPFlash (control) reporter plasmids. Twenty‐four hours posttransfection, SW480 cells were washed and lysed, and the luciferase activity was assayed (B). For RKO cells, which display normal canonical Wnt that needs to be activated by Wnt3a ligand (A), cells were serum starved (2%) for 10 h, and then Wnt3a (100 ng/mL) or vehicle was added to the medium to stimulate cells for 12 h period; the cells were then washed and lysed, and the luciferase activity was assayed. The activity in both cell types was normalized with respect to the activity of Renilla luciferase or with respect to the protein content in each sample. The data represent the means ± SEM from at least five independent assays. *** p < .001.

    Article Snippet: The β‐catenin transcriptional activity‐reporter plasmids pTOPFlash and pFOPFlash (control) were obtained from Upstate Biotechnology (Lake Placid, NY, USA).

    Techniques: Control, Transfection, Luciferase, Activity Assay